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Surefire Biosciences

SureFire Bio is dedicated to fulfilling all your Sanger sequencing needs, from acomprehensive line of high-quality reagents to expert instrument service coupled with anunprecedented level of support that provides greater value for your lab

Our advantages

QuantumSeq™ Sanger
sequencing reagents

Direct, drop-in substitutes for BigDye® Terminators, POP-7™ polymer and 5x Sequencing Buffer

Instrument
Service

Premium field service provided by highly skilled engineers at a fraction of the OEM cost

Exceptional Customer and
Technical Support

At SureFire Bio your FIRST CLASS customer experience is our number one priority!

QuantumSeq™ Reagents

Our unique QuantumSeq™ product line provides you with a complete solution of cost-effective alternatives, including QuantumDye™ Terminators, a direct substitute for BigDye™ Terminators, QuantumPOP™ 7, a direct substitute for POP-7™ polymer, QuantumDye 5x Buffer, a direct substitute for BigDye™ Terminator 5x Sequencing Buffer, and QuantumSAP, a direct substitute for ExoSAP-IT™ PCR Product Cleanup Reagent.

QuantumDye Terminators, QuantumPOP 7 Polymer, and QuantumDye 5x Buffer provide the same high-quality sequencing results with no changes in protocol, settings, spectral calibration or Dye Set.

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QuantumDye v3.1 Sequencing Kits

QuantumDye v3.1 Kits:

  • Yield uniform peak heights with extremely low background
  • Provide long sequence reads with high confidence scores
  • Generate results that are identical to BigDye® Terminator v3.1
QuantumDye dGTP v3.1 Sequencing Kits

QuantumDye dGTP v3.1 Kits:

  • Are optimized for sequencing through GT- and G-rich templates
  • Provide more efficient extension from the sequencing primer
  • Enable extension through difficult-to-sequence regions with confidence
  • Minimize early signal loss in G-rich samples
QuantumSAP™ PCR cleanup Reagent

QuantumSAP was developed as a cost-effective alternative to ExoSAP-IT™ for the removal of residual nucleotides and primers after PCR. QuantumSAP reagent contains two hydrolytic enzymes that inactivate the unincorporated reactants, allowing the PCR products to be used for cycle sequencing or genotyping without the need for further processing.

QuantumPOP 7 polymer

QuantumPOP™ 7 polymer is a pre-formulated separation matrix customized for DNA sequencing in capillary electrophoresis systems and is a direct substitute for POP-7 Polymer that provides the same high performance, but with greater value.

QuantumDye 5x Buffer

QuantumSeq™ 5x Sequencing Buffer was developed as a cost-effective alternative to BigDye™ Terminator v1.1 & v3.1 5x Sequencing Buffer for use in cycle sequencing applications. QuantumSeq 5x Sequencing Buffer is intended for use with QuantumSeq QuantumDye™ cycle sequencing kits to adjust the final reaction volume when using less cycle sequencing mix.

QuantumSeq 96-well Plate Sealers

QuantumSeq Plate Sealers are ideal for protecting samples from contamination or spillage in 96- or 384-well plates during shaking, incubation, and storage at -20°C, 4°C, or ambient temperature. Unlike similar products from other companies, no special tools are required for a secure, leakproof seal.

  • Resistant to cold and heat, from -20° C to 100° C
  • Removes cleanly with no adhesive residue
  • Will not tear when removed, even when stored at -20°C
  • Designed for easy, rapid application
  • Packaged in sets of 25
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Product Information

We prepared detailed brochure about our products and all specifications you are interested to know about it.

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About surefire bio

We are a new supplier focused exclusively on Sanger sequencing to provide you with a complete solution of cost-effective alternatives. We strive to provide Sanger sequencing labs with unmatched quality and customer support while preserving the integrity of your equipment and data with the goal of lowering your overall cost of sequencing.

Faq

Do I need to follow the QuantumDye protocol or can I use my current BigDye protocol without changes?
How much can I dilute QuantumDye?
How much does QuantumDye cost compared to BigDye?
Is a SureFire Bio service contract a direct replacement for my contract from the manufacturer?
What do service contracts cost from SureFire Bio?
Does SureFire use OEM parts to repair my DNA Sequencer?

Contact Us

We would love to hear from you.

You can contact us directly by phone at (888) 765 2097
or by email at contact@surefirebio.com.

For a quote or a free sample please fill the form below.

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