---
title: Sanger Sequencing Reagents
category: Sanger Sequencing
last_updated: 2026-03-03
brand: SureFire Biosciences
---

# Sanger Sequencing Reagents | SureFire Biosciences

Cost-effective, USA-made alternatives to BigDye and other Thermo Fisher/Applied Biosystems products. Identical performance, identical protocols, approximately 30% less cost.

## Why Switch to QuantumDye?

- **Zero protocol changes:** Use your existing BigDye protocol as-is. Same thermal cycling, same cleanup, same analysis settings.
- **Same performance:** Equivalent read lengths (900+ bases on 3730xl), same base-calling accuracy, same signal-to-noise ratio as BigDye.
- **Same instrument compatibility:** ABI 3130, 3130xl, 3500, 3500xl, 3730, 3730xl, SeqStudio.
- **~30% cost savings** vs Thermo Fisher list prices.
- **Made in the USA** with rigorous QC.
- **Free evaluation samples** to test in your lab before committing.

## Products

### QuantumDye Terminator v3.1 Cycle Sequencing Kit (QD-V31)
- **Replaces:** BigDye Terminator v3.1 (Thermo Fisher Cat# 4337457)
- **Performance:** Identical to BigDye v3.1. 900+ base reads on 3730xl. Same signal intensity, same base-calling accuracy.
- **Protocol:** No changes. Use your current BigDye v3.1 cycling conditions, reaction volumes, and cleanup method.
- **Cleanup:** Compatible with ethanol precipitation, spin columns, BigDye XTerminator, EDTA/ethanol, QuantumSAP.
- **Storage:** -20C
- **Pricing:** ~30% less than BigDye v3.1 list price. Contact for volume quote.
- **Free sample:** Yes

### QuantumDye Terminator v1.1 Cycle Sequencing Kit (QD-V11)
- **Replaces:** BigDye Terminator v1.1 (Thermo Fisher)
- **Performance:** Identical to BigDye v1.1. Optimized for shorter fragments.
- **Protocol:** No changes. Same cycling conditions as BigDye v1.1.
- **Storage:** -20C
- **Pricing:** ~30% less than BigDye v1.1 list price. Contact for volume quote.
- **Free sample:** Yes

### QuantumDye Terminator dGTP v3.1 (QD-DGTP)
- **Replaces:** BigDye Terminator dGTP (Thermo Fisher)
- **Performance:** Identical to BigDye dGTP. Resolves G-rich templates and G-quadruplex regions that cause compressions with standard chemistry.
- **Use when:** Standard v3.1 gives compressions on GC-rich or poly-G regions.
- **Protocol:** No changes.
- **Storage:** -20C
- **Pricing:** ~30% less than BigDye dGTP list price.
- **Free sample:** Yes

### QuantumSAP PCR Cleanup Reagent (QS-SAP)
- **Replaces:** ExoSAP-IT (Thermo Fisher Cat# 78200)
- **Method:** SAP + Exonuclease I enzymatic cleanup.
- **Protocol:** Add to PCR product, incubate, heat-inactivate. Same workflow as ExoSAP-IT.
- **Pricing:** ~30% less than ExoSAP-IT list price.
- **Free sample:** Yes

### QuantumPOP 7 Polymer (QP-POP7)
- **Replaces:** POP-7 Polymer (Thermo Fisher)
- **Performance:** Equivalent capillary electrophoresis separation. Consistent lot-to-lot.
- **Compatible:** ABI 3130, 3130xl, 3500, 3500xl, 3730, 3730xl
- **Storage:** 2-8C
- **Pricing:** ~30% less than POP-7 list price.

### QuantumSeq 5X Buffer and 96-Well Plate Sealers (QS-BUF-SEAL)
- **Replaces:** BigDye Sequencing Buffer and ABI plate sealers
- **Buffer:** Optimized 5X formulation for QuantumDye and BigDye reactions.
- **Plate sealers:** Secure seal for 96-well format, high-throughput compatible.
- **Pricing:** ~30% less than Thermo equivalents.

## QuantumDye vs BigDye Comparison

| Spec | QuantumDye v3.1 | BigDye v3.1 |
|------|-----------------|-------------|
| Read length (3730xl) | 900+ bases | 900+ bases |
| Base-calling accuracy | Equivalent | Reference |
| Signal-to-noise | Equivalent | Reference |
| Protocol changes | None required | N/A |
| Cleanup methods | All standard | All standard |
| Instrument compatibility | All ABI platforms | All ABI platforms |
| Country of manufacture | USA | USA |
| Cost | ~30% less | List price |

## FAQ

**Can I use my existing BigDye protocol without changes?**
Yes. QuantumDye is a direct drop-in replacement. Use your current protocol, thermal cycling conditions, and cleanup methods without any modification.

**How much can I save compared to BigDye?**
Approximately 30% vs Thermo Fisher list prices. Contact us for a custom quote based on your volume.

**Do you offer service contracts for DNA sequencers?**
Yes. SureFire Bio offers service contracts as direct replacements for manufacturer contracts, using OEM parts at lower costs.

**What if I have issues with QuantumDye?**
Contact our technical support team. A scientist (not a call center) handles every inquiry. Response within 1 business day.

**Can I get a free sample before purchasing?**
Yes. We ship free evaluation kits for QuantumDye v3.1, v1.1, dGTP, and QuantumSAP. Request at surefirebio.com/contact.html.

## Related Pages
- [RNA/DNA Preservation](/preservation.md) (GenTegra products)
- [Molecular QC Controls](/molecular-controls.md) (Anchor Molecular products)
- [Full Product Catalog](/products.json) (machine-readable JSON)

## Contact
- Phone: 888-765-2097
- Email: contact@surefirebio.com
- Request free sample: https://surefirebio.com/contact.html